1999 ), or protein–protein interactions using two-hybrid technologies (Rain et al. 2001 ), protein subcellular localization using reporter genes (Whiteley et al.
1996 ), protein expression using proteome chips (Zhu et al. Significant functional information can be acquired on a genome scale by analyzing, among other things, gene expression using DNA microarrays (Schena et al. The conversion of these sequence data into meaningful biologic information, by performing whole-genome experimental investigations, is therefore an important scientific challenge.
The two complete meningococcal sequences contain an average of 2100 predicted coding regions, of which half were assigned no biologic role, whereas most of the remaining half were assigned roles that await experimental validation (Parkhill et al.
This can be illustrated by the example of Neisseria meningitidis, a human pathogen that is one of the leading causes of fatal sepsis and meningitis worldwide (Tikhomirov et al. The ongoing accumulation of complete genome sequences in the public databases made it clear that a genome sequencing project is a small step in the understanding of the biology of an organism. Our work provides a valuable and lasting resource that may lead to a global map of gene function in N. We show that all the genes we identified, including four previously uncharacterized, were important for the synthesis of the polysialic acid capsule or the lipooligosaccharide (LOS), suggesting that these are likely to be the only meningococcal attributes necessary for serum resistance. This was confirmed by a whole-genome identification of the genes required for resistance to complement-mediated lysis, a key to meningococcal virulence. The sequencing results for the first half of the 4548 mutants composing the library suggested that we have mutations in 80%–90% of N. Determining the phenotypic effect of gene disruption being a fundamental approach to understanding gene function, we used high-density signature-tagged transposon mutagenesis, followed by a large-scale sequencing of the transposon insertion sites, to construct a genome-wide collection of mutants. Taking all things into consideration, VimR is a streamlined and useful text editing solution for development purposes that manages to improve the whole Vim experience, with a useful file browser and a quick search feature, as well as with a modern user interface stitched on top.The biologic role of a majority of the Neisseria meningitidis 2100 predicted coding regions is still to be assigned or experimentally confirmed. Easy to use, small, and efficient Vim expansion In a successful attempt to improve the whole Vim experience, VimR comes with a new file browser that makes it easy for you to search for, find, and view your files.įurthermore, if the file browser doesn’t help you find the exact file you are looking for, you can always use VimR’s built-in Open Quickly search bar that makes it easy for you to rapidly open files via Xcode or TextMate, a text editor that many consider to be the one thing that’s missing from OS X’s development toolkit repertoire. Brings to the table two important new features for Vim, namely a file browser and a quick search function To start off, if you are a Vim fan you will be delighted to hear that VimR hasn’t lost its way from the true Vim philosophy by remaining very customizable and extensible and, thus, it enables you to use all your favorite plugins and scripts. VimR is an macOS standalone application that uses the Vim engine neatly tucked within a straightforward and easy to use interface and it seems to be the logical next step for everyone who loves and uses the Vim text editor. Keeps all of Vim's good parts and improves the overall experience Despite the fact that nowadays most computer based development work is done using modern and fully featured applications, you will sometimes, often or not, need an old-school text editor for certain tasks.Ī free, very customizable, thoroughly documented, and probably one of the most influential text editors out there is Vim.
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